BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader

Absorbance Specifications Light Source Xenon flash lamp Wavelength Selection Monochromator Wavelength Range 230 to 999 nm, in 1 nm increments Bandpass 2 nm (230 to 285 nm), 4 nm (> 285 nm) Dynamic Range 0 to 4.0 OD Photometric Resolution 0.0001 OD Pathlength Correction Yes Photometric Accuracy < 1% at 2.0 OD typical < 3% at 3.0 OD typical Photometric Repeatability < 0.5% at 2.0 OD typical Fluorescence Intensity Specifications Light Source Tungsten halogen lamp Wavelength Selection Double grating monochromators (top/bottom) and deep blocking filters/dichroic mirrors (top) Wavelength Range Monochromators: 250 to 850 nm Filters: 200 to 700 nm (850 nm option) Dynamic Range Monochromators: 5 decades Filters/Mirrors: > 6 decades Sensitivity Top Monochromator: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate) Bottom Monochromator: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate) Luminescence Specifications Wavelength Range 300 to 700 nm Dynamic Range < 6 decades Sensitivity 10 amol ATP typical (flash) Fluorescence Polarization Specifications Light Source Tungsten halogen Wavelength Selection Deep blocking filters/dichroic mirrors (top) Wavelength Range 400 to 700 nm (320 to 850 nm option) Sensitivity 3 mP at 1 nm fluorescein typical Time-Resolved Fluorescence Specifications Light Source High energy xenon flash lamp Wavelength Range 200 to 700 nm (850 nm option) Sensitivity Europium 60 fM typical with filters (6 amol/well in 384-well plate)